Construction of an Overlapping Clone Library

image credit: U.S. Department of Energy Human Genome Program, http://www.ornl.gov/hgmis.

caption: A collection of clones of chromosomal DNA, called a library, has no obvious order indicating the original positions of the cloned pieces on the uncut chromosome. To establish that two particular clones are adjacent to each other in the genome, libraries of clones containing partly overlapping regions must be constructed. These clone libraries are ordered by dividing the inserts into smaller fragments and determining which clones share common DNA sequences.

For more on the science behind the Genomes to Life Program, see Website.

This image originally appeared in the 1992 U.S. DOE Primer on Molecular Genetics.

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